Role of Hyalin-like Protein in Gliding and Biofilm Formation by <i>Capnocytophaga Ochracea</i>

Capnocytophaga ochracea possesses a type-IX secretion system that exports proteins which have gliding motility-associated C-terminal (CTD) domain. This is found in several species of the Bacteroidetes phylum. Hyalin, large protein encoded by Coch_0033 C. ATCC 27872, has CTD domain and posited to be involved quorum sensing according database Kyoto Encyclopedia Genes Genomes. suggests it plays role biofilm formation via interbacterial communication. The aim this study was investigate potential hyalin-like coded gene ochracea. A protein-deficient mutant strain ochracea, designated WR-1, constructed through insertion ermF-ermAM cassette into target gene. spreading feature at edge colony lost strain. Crystal violet confocal laser scanning microscopy revealed no difference between quantity organized wild-type These data suggest indeed activity.